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black microtiter non binding assay plates  (Greiner Bio)


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    Greiner Bio black microtiter non binding assay plates
    Black Microtiter Non Binding Assay Plates, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 97/100, based on 755 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/black+microtiter+non+binding+assay+plates/pm40848739-47-10-26?v=Greiner+Bio
    Average 97 stars, based on 755 article reviews
    black microtiter non binding assay plates - by Bioz Stars, 2026-08
    97/100 stars

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    Characterization of a glycolate-responsive in vitro transcription-based biosensor module. A: The ROSALIND system is based on the controlled expression of the 3WJdB RNA aptamer and the correlating fluorescence signal of the 3WJdB :DFHBI-1T complex. Expression is regulated by a transcriptional repressor that binds to an operator sequence downstream of a T7 promoter. The repression is lifted in a dose-responsive manner by binding of an effector molecule to the aTF. This system allows faster and cheaper sample measurement in <t>microtiter</t> plates than analysis by LC-MS, but its precision is yet unknown. Time estimates refer to glycolate quantification. B, C: The dose-response curve of the GlcR sensor module to 100 nM to 100 mM glycolate (16 h time point) shows an operational range from 10 µM to 20 mM glycolate with an excellent correlation between 16 µM and 8 mM glycolate and a 10-fold dynamic range. Note that IVT biosensing reactions are highly time-sensitive (Supplementary Figure 5). D: Promiscuity assay of GlcR shows a low-level dose response to DL-lactate and DL-glycerate. Raw fluorescence data are standardized to MEF (µM fluorescein). Data are the mean of n =3 technical replicates ± s.d. IVT output without effector molecule and without MGlcR is shown as horizontal dotted lines.
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    black polystyrene 384-well microtiter plate with a non-binding surface  (Corning Life Sciences)
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    Characterization of a glycolate-responsive in vitro transcription-based biosensor module. A: The ROSALIND system is based on the controlled expression of the 3WJdB RNA aptamer and the correlating fluorescence signal of the 3WJdB :DFHBI-1T complex. Expression is regulated by a transcriptional repressor that binds to an operator sequence downstream of a T7 promoter. The repression is lifted in a dose-responsive manner by binding of an effector molecule to the aTF. This system allows faster and cheaper sample measurement in <t>microtiter</t> plates than analysis by LC-MS, but its precision is yet unknown. Time estimates refer to glycolate quantification. B, C: The dose-response curve of the GlcR sensor module to 100 nM to 100 mM glycolate (16 h time point) shows an operational range from 10 µM to 20 mM glycolate with an excellent correlation between 16 µM and 8 mM glycolate and a 10-fold dynamic range. Note that IVT biosensing reactions are highly time-sensitive (Supplementary Figure 5). D: Promiscuity assay of GlcR shows a low-level dose response to DL-lactate and DL-glycerate. Raw fluorescence data are standardized to MEF (µM fluorescein). Data are the mean of n =3 technical replicates ± s.d. IVT output without effector molecule and without MGlcR is shown as horizontal dotted lines.
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    Characterization of a glycolate-responsive in vitro transcription-based biosensor module. A: The ROSALIND system is based on the controlled expression of the 3WJdB RNA aptamer and the correlating fluorescence signal of the 3WJdB :DFHBI-1T complex. Expression is regulated by a transcriptional repressor that binds to an operator sequence downstream of a T7 promoter. The repression is lifted in a dose-responsive manner by binding of an effector molecule to the aTF. This system allows faster and cheaper sample measurement in microtiter plates than analysis by LC-MS, but its precision is yet unknown. Time estimates refer to glycolate quantification. B, C: The dose-response curve of the GlcR sensor module to 100 nM to 100 mM glycolate (16 h time point) shows an operational range from 10 µM to 20 mM glycolate with an excellent correlation between 16 µM and 8 mM glycolate and a 10-fold dynamic range. Note that IVT biosensing reactions are highly time-sensitive (Supplementary Figure 5). D: Promiscuity assay of GlcR shows a low-level dose response to DL-lactate and DL-glycerate. Raw fluorescence data are standardized to MEF (µM fluorescein). Data are the mean of n =3 technical replicates ± s.d. IVT output without effector molecule and without MGlcR is shown as horizontal dotted lines.

    Journal: bioRxiv

    Article Title: In vitro transcription-based biosensing of glycolate for prototyping of a complex enzyme cascade

    doi: 10.1101/2024.04.26.591264

    Figure Lengend Snippet: Characterization of a glycolate-responsive in vitro transcription-based biosensor module. A: The ROSALIND system is based on the controlled expression of the 3WJdB RNA aptamer and the correlating fluorescence signal of the 3WJdB :DFHBI-1T complex. Expression is regulated by a transcriptional repressor that binds to an operator sequence downstream of a T7 promoter. The repression is lifted in a dose-responsive manner by binding of an effector molecule to the aTF. This system allows faster and cheaper sample measurement in microtiter plates than analysis by LC-MS, but its precision is yet unknown. Time estimates refer to glycolate quantification. B, C: The dose-response curve of the GlcR sensor module to 100 nM to 100 mM glycolate (16 h time point) shows an operational range from 10 µM to 20 mM glycolate with an excellent correlation between 16 µM and 8 mM glycolate and a 10-fold dynamic range. Note that IVT biosensing reactions are highly time-sensitive (Supplementary Figure 5). D: Promiscuity assay of GlcR shows a low-level dose response to DL-lactate and DL-glycerate. Raw fluorescence data are standardized to MEF (µM fluorescein). Data are the mean of n =3 technical replicates ± s.d. IVT output without effector molecule and without MGlcR is shown as horizontal dotted lines.

    Article Snippet: The reactions were mixed by pipetting and 3x 20 μL were immediately transferred into a 384-well, black, optically clear, flat-bottom, non-binding microtiter plate (Greiner Bio-One, Kremsmünster, Austria; catalog no.: 781906).

    Techniques: In Vitro, Expressing, Fluorescence, Sequencing, Binding Assay, Liquid Chromatography with Mass Spectroscopy